School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nationâ€™s top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.General Purpose (see percentages below) Under the direction of the principle investigators the individual will be responsible for retrieval, processing, and cataloging human muscle biopsy samples obtained from the OR from rotator cuff tear/injury patients, as well as muscle tissues obtained from mice following rotator cuff surgery/injury in our animal colony. Responsibilities also include the maintenance, management of associated paperwork, and ordering of sufficient inventory of material, supplies, and equipment for the performance of duties and laboratory projects. Responsibilities for awareness and implementation of laboratory safety procedures along with the making of; stock solutions, culture media, chemicals, and reagents. Responsibilities for the maintenance of laboratory equipment, training of lab personnel for fundamental skills needed to perform various projects, and cleanliness of the lab. The individual will be expected to assist with various rotator cuff injury projects in the lab through conducting fluorescence activated cell sorting (FACS)/flow cytometry, fundamental gene and protein expression analysis, cell culture, tissue sectioning, immunochemistry (IHC), immunofluorescence (IF), microscopy, and tissue morphometric analysis. The individual will be expected to conduct data quantification and preparation of; graphs, charts, and figures for scientific publication. There will also be an expectation to maintain database for raw data and organize/prepare data for analysis by the principal investigators. Previous experience particularly with FACS/flow cytometry and tissue analysis is preferred. Previous experience with/or willingness to learn muscle biology desired. Ability to work in a lab team environment with a diverse group of personnel is essential.
Typical Assignments and Responsibilities (see percentages below)In accordance with broadly-outlined objectives, requirements and approaches above, the individual will set-up and conduct technical procedures, non-routine and complex in nature, and operate state-of-the-art equipment or instruments. With latitude for modifying methods and techniques, the individual will analyze data, prepare reports, present data at laboratory meetings, ensure appropriate documentation of work and experiments, and make recommendations. They will directly report to the principal investigators through scheduled mandatory meetings. The will be responsible for ordering laboratory supplies and interacting with vendors. The individual will work with computers, scientific software, Excel, Word, etc. ESSENTIAL TASKS Specific Responsibilities:
50% Retrieval, processing, cataloging, and analyzing human muscle biopsy samples. Work in an independent manner to retrieve control and torn rotator cuff muscle biopsies from the OR, maintain records with appropriate software, and analyze tissue samples. Specific responsibilities will include tissue digestions, cell isolation, cell culture, FACS/flow cytometry, muscle biopsy tissue fixation, processing, and embedding for frozen/paraffin tissue sectioning, IHC, IF, and real-time qPCR and protein analysis (Western) from isolated tissues and/or cells.
30% Mouse rotator cuff surgeries, processing, cataloging, and analysis of murine muscle tissue. Work in an independent manner to set-up and perform murine rotator cuff injuries and subsequent analysis. Specific responsibilities will include tissue digestions, cell isolation, cell culture, FACS/flow cytometry, muscle biopsy tissue fixation, processing, and embedding for frozen/paraffin tissue sectioning, IHC, IF, and real-time qPCR and protein analysis (Western) from isolated tissues and/or cells.
10% General laboratory maintenance. Order supplies and work closely with departmental administrative office staff on purchasing and account issues. Prepare common reagents, train other laboratory workers, graduate students, and postdoctoral fellows in fundamental laboratory techniques and equipment use. Provide support and technical assistance on experimental problems that may arise during the course of their experiments. Maintain functionality of lab equipment, as well as the overall organization of the lab, and comply with all institutional laboratory safety procedures. Maintain careful laboratory notebooks, organize raw data, analyze data, and assist with the implementation of new protocols.
10% Research Analysis, Presentation, Searches. Analyze and present findings to PI and in laboratory meetings. Develop graphs, charts, and figures for scientific publication.
Non-essential tasks: Not Applicable
CONTACTS: Daily interactions with Principal Investigators as well as other administrative and laboratory staff.
Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.
None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.
Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.
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